bmprib antibody  (Thermo Fisher)

Journal: PLoS ONE

Article Title: BMP4 Increases the Expression of TRPC and Basal [Ca 2+ ] i via the p38MAPK and ERK1/2 Pathways Independent of BMPRII in PASMCs

doi: 10.1371/journal.pone.0112695

Figure Lengend Snippet: Representative blots (A) and normalized bar graphs (B) of BMPRII, ActRIIa, ActRIIb, ALK2, BMPRIa and BMPRIb protein as detected by Western blotting in PASMCs treated with 50 ng/ml BMP4 for 60 h. Bar graph shows mean protein expression for BMPRII, ActRIIa, ActRIIb, ALK2, BMPRIa and BMPRIb protein relative to α-actin (n = 4 in each group; * P <0.05 vs. vehicle control). Bar values are as means ± SEM.

Article Snippet: All antibodies were obtained as follow: TRPC1, 6 antibodies (abcam), TRPC4 antibody (Alomone Laboratories), phospho-Smad1/5/8, phospho-ERK1/2, phospho-p38MAPK, ERK1/2, Smad5 and p38MAPK antibodies (Cell Signaling Technology), BMPRIa antibody (Proteintech Group), BMPRIb antibody (Invitrogen), ALK2 antibody (Novus), BMPRII antibody (BD), ActR2a and ActR2b antibodies (Abbiotec), α-actin antibody (Sigma-Aldrich).

Techniques: Western Blot, Expressing

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Both the two cell types showed typical spindle-shaped morphology (×40) in regular cultures ( left ). After one week of osteogenic differentiation, a positive AP staining was detected in either dermal BMPRIB + cells or BMSCs ( middle ). Moreover, they were stained positive for AR after two weeks’ induction ( Right ). In contrast, only very week AP staining or AR staining was observed when they were cultured in non-osteogenic conditions (images not shown). BMPRIB + cells : morphogenetic protein receptor IB positive cells, BMSCs : bone marrow mesenchymal stromal cells, AP : alkaline phosphatase, AR : Alizarin Red.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Staining, Cell Culture

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Under osteogenic conditions, dermal BMPRIB + cells and BMSCs showed a similar AP production at one week (above , left) and calcium nodules formations at two week (above , right) . For osteogenic gene expression on mRNA level at day 14, dermal BMPRIB + cells produced a significantly higher OCN (a late osteogenic marker) expression than BMSCs, while transcript levels for Runx2 (an early osteogenic marker)and OPN (an intermediate osteogenic marker)were not significantly different between two groups (below) .Bars represent mean ± SD of n = 3 donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells; OPN : osteopontin; OCN : osteocalcin.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Gene Expression, Produced, Marker, Expressing

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Phenotypic analysis of C57BL/6 BMPRIB + cells and BMSCs before and after osteogenic differentiation by flow cytometry.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Cytometry

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Balb/c splenocytes were co-cultured with four mitotically inactivated different cell isolates at passage three at a cell ratio of 1:1for 5 days. Autologous mitotically inactivated splenocytes were set as negative control, while C57BL/6 mitotically inactivated splenocytes were set as positive control. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells; NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Cell Culture, Negative Control, Positive Control

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Four mitotically inactivated different cell isolates were added to allogeneic MLR at the numbers ranging from 10000, 50000, and 100000 cells per well. Autologous inactivated splenocytes were set as negative control. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells. NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Negative Control

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Following co-culture with allogeneic MLR for 5 days, undifferentiated BMPRIB + cells upregulated the iNOS expression drastically above the basal level, while IDO production was found to be upregulated greatly in osteogenic BMPRIB + cells. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; MLR : mixed lymphocyte reaction; iNOS : inducible NO synthase; IDO : indoleamine 2,3-dioxygenase; NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Co-Culture Assay, Expressing

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Mitotically inactivated undifferentiated C57BL/6 BMPRIB + cells (1×10 5 cells) were co-cultured with Responder Balb/c splenocytes and stimulator C57BL/6 splenocytes at a 1:1:1 ratio in 200μl culture medium. Cells were treated with 1400W (0.1mM, 1mM) or 1-MT (1mM) for 5 days and proliferation was performed respectively. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; 1400W : N-[[3-(aminomethyl)phenyl]methyl]-ethanimidamide; 1-MT :1-methyl tryptophan. NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Mitotically inactivated osteogenic differentiated C57BL/6 BMPRIB + cells (1×10 5 cells) were co-cultured with Responder Balb/c splenocytes and stimulator C57BL/6 splenocytes at a 1:1:1 ratio in 200μl culture medium. Cells were treated with 1-MT (0.1mM, 1mM) or 1400W (1mM) for 5 days and proliferation was performed respectively. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; 1400W : N-[[3-(aminomethyl)phenyl]methyl]-ethanimidamide; 1-MT : 1-methyl tryptophan. NS : no significant.

Article Snippet: Appropriate 1X 10 7 /ml FBs were incubated with saturating concentrations (1:100dilution) of primary rabbit anti-mouse IgG monoclonal antibody BMPRIB (Santa cruz) for 20 minutes on ice, and then the cells were magnetically labeled with Anti-rabbit IgG microbeads.

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Both the two cell types showed typical spindle-shaped morphology (×40) in regular cultures ( left ). After one week of osteogenic differentiation, a positive AP staining was detected in either dermal BMPRIB + cells or BMSCs ( middle ). Moreover, they were stained positive for AR after two weeks’ induction ( Right ). In contrast, only very week AP staining or AR staining was observed when they were cultured in non-osteogenic conditions (images not shown). BMPRIB + cells : morphogenetic protein receptor IB positive cells, BMSCs : bone marrow mesenchymal stromal cells, AP : alkaline phosphatase, AR : Alizarin Red.

Article Snippet: Briefly, cells were initially incubated with saturated (1:100) non-labeled anti-BMPRIB rabbit monoclonal antibody (Santa cruz) on ice for 20 min. After three washes, FITC-conjugated goat anti-rabbit IgG (1:100; abcam,Cambridge, UK)were used to amplify the signals.

Techniques: Staining, Cell Culture

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Under osteogenic conditions, dermal BMPRIB + cells and BMSCs showed a similar AP production at one week (above , left) and calcium nodules formations at two week (above , right) . For osteogenic gene expression on mRNA level at day 14, dermal BMPRIB + cells produced a significantly higher OCN (a late osteogenic marker) expression than BMSCs, while transcript levels for Runx2 (an early osteogenic marker)and OPN (an intermediate osteogenic marker)were not significantly different between two groups (below) .Bars represent mean ± SD of n = 3 donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells; OPN : osteopontin; OCN : osteocalcin.

Article Snippet: Briefly, cells were initially incubated with saturated (1:100) non-labeled anti-BMPRIB rabbit monoclonal antibody (Santa cruz) on ice for 20 min. After three washes, FITC-conjugated goat anti-rabbit IgG (1:100; abcam,Cambridge, UK)were used to amplify the signals.

Techniques: Gene Expression, Produced, Marker, Expressing

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Phenotypic analysis of C57BL/6 BMPRIB + cells and BMSCs before and after osteogenic differentiation by flow cytometry.

Article Snippet: Briefly, cells were initially incubated with saturated (1:100) non-labeled anti-BMPRIB rabbit monoclonal antibody (Santa cruz) on ice for 20 min. After three washes, FITC-conjugated goat anti-rabbit IgG (1:100; abcam,Cambridge, UK)were used to amplify the signals.

Techniques: Cytometry

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Balb/c splenocytes were co-cultured with four mitotically inactivated different cell isolates at passage three at a cell ratio of 1:1for 5 days. Autologous mitotically inactivated splenocytes were set as negative control, while C57BL/6 mitotically inactivated splenocytes were set as positive control. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells; NS : no significant.

Article Snippet: Briefly, cells were initially incubated with saturated (1:100) non-labeled anti-BMPRIB rabbit monoclonal antibody (Santa cruz) on ice for 20 min. After three washes, FITC-conjugated goat anti-rabbit IgG (1:100; abcam,Cambridge, UK)were used to amplify the signals.

Techniques: Cell Culture, Negative Control, Positive Control

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Four mitotically inactivated different cell isolates were added to allogeneic MLR at the numbers ranging from 10000, 50000, and 100000 cells per well. Autologous inactivated splenocytes were set as negative control. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; BMSCs : bone marrow mesenchymal stromal cells. NS : no significant.

Article Snippet: Briefly, cells were initially incubated with saturated (1:100) non-labeled anti-BMPRIB rabbit monoclonal antibody (Santa cruz) on ice for 20 min. After three washes, FITC-conjugated goat anti-rabbit IgG (1:100; abcam,Cambridge, UK)were used to amplify the signals.

Techniques: Negative Control

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Following co-culture with allogeneic MLR for 5 days, undifferentiated BMPRIB + cells upregulated the iNOS expression drastically above the basal level, while IDO production was found to be upregulated greatly in osteogenic BMPRIB + cells. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; MLR : mixed lymphocyte reaction; iNOS : inducible NO synthase; IDO : indoleamine 2,3-dioxygenase; NS : no significant.

Article Snippet: Briefly, cells were initially incubated with saturated (1:100) non-labeled anti-BMPRIB rabbit monoclonal antibody (Santa cruz) on ice for 20 min. After three washes, FITC-conjugated goat anti-rabbit IgG (1:100; abcam,Cambridge, UK)were used to amplify the signals.

Techniques: Co-Culture Assay, Expressing

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Mitotically inactivated undifferentiated C57BL/6 BMPRIB + cells (1×10 5 cells) were co-cultured with Responder Balb/c splenocytes and stimulator C57BL/6 splenocytes at a 1:1:1 ratio in 200μl culture medium. Cells were treated with 1400W (0.1mM, 1mM) or 1-MT (1mM) for 5 days and proliferation was performed respectively. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; 1400W : N-[[3-(aminomethyl)phenyl]methyl]-ethanimidamide; 1-MT :1-methyl tryptophan. NS : no significant.

Article Snippet: Briefly, cells were initially incubated with saturated (1:100) non-labeled anti-BMPRIB rabbit monoclonal antibody (Santa cruz) on ice for 20 min. After three washes, FITC-conjugated goat anti-rabbit IgG (1:100; abcam,Cambridge, UK)were used to amplify the signals.

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: The Immunologic Properties of Bone Morphogenic Protein Receptor IB Positive Subpopulation before and after Osteogenic Differentiation in Mouse Dermis

doi: 10.1371/journal.pone.0161785

Figure Lengend Snippet: Mitotically inactivated osteogenic differentiated C57BL/6 BMPRIB + cells (1×10 5 cells) were co-cultured with Responder Balb/c splenocytes and stimulator C57BL/6 splenocytes at a 1:1:1 ratio in 200μl culture medium. Cells were treated with 1-MT (0.1mM, 1mM) or 1400W (1mM) for 5 days and proliferation was performed respectively. Bars represent mean ± SD of n = 3donor samples. ＊ p<0.05. BMPRIB + cells : morphogenetic protein receptor IB positive cells; 1400W : N-[[3-(aminomethyl)phenyl]methyl]-ethanimidamide; 1-MT : 1-methyl tryptophan. NS : no significant.

Article Snippet: Briefly, cells were initially incubated with saturated (1:100) non-labeled anti-BMPRIB rabbit monoclonal antibody (Santa cruz) on ice for 20 min. After three washes, FITC-conjugated goat anti-rabbit IgG (1:100; abcam,Cambridge, UK)were used to amplify the signals.

Techniques: Cell Culture

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Characterization of BmprIB+ dermal cells. (A): Immunohistochemical staining of BmprIB in human foreskin. (B): Percentage evaluation of BmprIB expression in freshly isolated human dermal cells by flow cytometry. Histograms of BmprIB expression (left; 3.5% ± 0.4%) and isotype control (right). (C): Cell morphology under phase‐contrast microscopy. (D): The proliferative potential was assessed with the Alamar Blue assay in usDCs and BmprIB+ cells. Cell numbers were quantified by the absorbance wavelength at 570 nm using a spectrophotometer at 24, 48, 72, and 96 hours. Data represented the mean of three individuals and each of them was the mean of triplicate experiments. The osteogenic potential of BmprIB+ cells was demonstrated by ALP staining (E) and ARS staining (F) . (G): The mRNA expression levels of ALP (at day 7) and OCN , OPN , and BSP (at day 21) were analyzed by real‐time polymerase chain reaction after osteogenic induction. Data represent the mean ± SD ( n = 3). ∗, p < .05 indicates statistical significance. Scale bars = 50 µm. Abbreviations: ALP, alkaline phosphatase; ARS, alizarin red S stain; BmprIB, bone morphogenetic protein receptor type IB; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Immunohistochemical staining, Staining, Expressing, Isolation, Flow Cytometry, Control, Microscopy, Alamar Blue Assay, Spectrophotometry, Real-time Polymerase Chain Reaction

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Dermal cell/coral scaffold compatibility. (A): Gross view of a coral scaffold with the size of 4‐mm diameter by 1‐mm thickness (top panel); two‐dimensional (left) and three‐dimensional images (right) of a coral scaffold by µCT scanning (bottom panel). (B): The cell proliferation analysis of the BmprIB+ cells and usDCs on the coral scaffold was assessed with the Alamar Blue assay at 12, 24, 36, 48, 60, 72, 84, and 96 hours. Data represent the mean of three individuals and each of them was the mean of triplicate experiments. (C): Scanning electron microscopy evaluation of the adherence and matrix deposition of BmprIB+ cells on the coral scaffold 1, 3, and 7 days after seeding. Scale bar = 20 µm. The coral alone without cell seeding served as a control. Scale bar = 100 µm. (D): Osteogenic potential of BmprIB+ cells and usDCs on the coral scaffold was evaluated according to the ALP activity and osteocalcin content at days 1, 3, 7, 14, 21, and 28 after osteogenic induction, respectively. Data represent the mean of three individuals and each of them was the mean of triplicate experiments. Abbreviations: 2D, two‐dimensional; ALP, alkaline phosphatase; BmprIB, bone morphogenetic protein receptor type IB; μCT, microcomputed tomography; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Alamar Blue Assay, Electron Microscopy, Control, Activity Assay, Tomography

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Evaluation of early‐stage osteogenic reconstruction. (A): A full‐thickness defect 4 mm in diameter was created by a hollow drill on the right side of the calvarial bone and repaired by coral alone, usDCs/coral, or BmprIB+ cells per coral in each group (top panel). Representative images of two‐dimensional (left) and three dimensional (right) µCT scanning were taken 24 hours after the surgery (bottom panel). (B): Histological analyses including H&E staining and Masson trichrome staining were used to evaluate new bone formation 6 weeks postimplantation. The black arrows indicate both ends of the defect. Scale bar = 1 mm. (C): Immunostaining was used to evaluate osteogenic marker expression, including osteocalcin, osteopontin, bone sialoprotein, and vascular marker CD31 in newly formed bone. Scale bar = 50 µm. (D): Histomorphometric analysis was used to quantify the percentage of positive staining area or vascular count. Data represent the average ± SD ( n = 6). ∗, p < .05 indicates statistical significance. Abbreviations: BmprIB, bone morphogenetic protein receptor type IB; H&E, hematoxylin and eosin; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Staining, Immunostaining, Marker, Expressing

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Microcomputed tomography (µCT) evaluation of late‐stage osteogenic reconstruction. (A): The defect was repaired by engineered bone on the right side of the mouse cranium 24 weeks postimplantation. Black arrows mark the repaired defect. (B): Representative three‐dimensional µCT images of parietal bones from each group 24 weeks postimplantation. Scale bar = 5 mm (left) and 1 mm (right). (C): The opaque percentage of each group was evaluated. (D): Other important bone structure parameters, such as BV (mm 3 ), BV/TV ratio (%), the Tb.N (1/mm), the Tb.Th (mm), the Tb.Sp, and Conn.D, were compared among these three implant groups with coral, usDCs/coral, and BmprIB/coral. Data represent the mean ± SD ( n = 6). ∗, p < .05 indicates statistical significance. Abbreviations: BmprIB, bone morphogenetic protein receptor type IB; BV, bone volume; BV/TV, ratio of bone volume to total volume; Conn.D, connectivity density; Tb.N, number of trabeculae; Tb.Sp, trabecular spacing; Tb.Th, thickness of the trabecular structure; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Tomography

Journal: Stem Cells Translational Medicine

Article Title: A Selective Cell Population from Dermis Strengthens Bone Regeneration

doi: 10.5966/sctm.2015-0426

Figure Lengend Snippet: Histological evaluation of late‐stage osteogenic reconstruction. (A): Goldner trichrome staining was used for plastic‐embedded, undecalcified, 24‐week regenerated bone. Mature bone matrix stained green and immature new bone matrix stained red. (B): Hematoxylin and eosin staining was also used to evaluate 24‐week regenerated bone. The black arrows indicate both ends of the defect. Scale bar = 1 mm. Abbreviations: BmprIB, bone morphogenetic protein receptor type IB; usDC, unsorted dermal cell.

Article Snippet: For magnetic‐activated cell sorting, the cell suspensions were centrifuged and resuspended in phosphate‐buffered saline (PBS; Sigma‐Aldrich) containing 0.5% bovine serum albumin (BSA; Sigma‐Aldrich), labeled with phycoerythrin (PE)‐conjugated anti‐human BmprIB antibody (FAB5051P; R&D Systems, Minneapolis, MN, https://www.rndsystems.com ), and further incubated with anti‐PE microbeads (catalog no. 130‐048‐801; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com ).

Techniques: Staining

Journal: Scientific Reports

Article Title: Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation

doi: 10.1038/srep23670

Figure Lengend Snippet: Sections of the molar of E18 mouse were immunostained for GDF5 ( a ), BMPRIA ( b ), BMPRIB ( c ), and BMPRII ( d ). Scale bars: 200 μm. Abbreviations: B, Buccal side; L, Lingual side.

Article Snippet: Solution (Polysciences, PA, USA) for 5 min for antigen activation, followed by incubation in 5% BSA/PBS for 1 h. After incubation with the primary antibodies for BMPRIA (sc-20736; Santa Cruz Biotechnology, CA, USA), BMPRIB (sc-25455; Santa Cruz Biotechnology), or BMPRII (MABD171; Merck Millipore, MA, USA), expression was detected using Alexa488- or Alexa594-conjugated secondary antibodies (Life Technologies).

Techniques:

Journal: Scientific Reports

Article Title: Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation

doi: 10.1038/srep23670

Figure Lengend Snippet: SF2 cells were cultured with BMP2, and the expression of AMEL and AMBN was analysed by real-time PCR ( a ), whereas the expression of BMPRIA, BMPRIB, and BMPRII was determined by RT-PCR ( b ). The expression of all genes analysed was normalised to the expression of GAPDH as an internal control.

Article Snippet: Solution (Polysciences, PA, USA) for 5 min for antigen activation, followed by incubation in 5% BSA/PBS for 1 h. After incubation with the primary antibodies for BMPRIA (sc-20736; Santa Cruz Biotechnology, CA, USA), BMPRIB (sc-25455; Santa Cruz Biotechnology), or BMPRII (MABD171; Merck Millipore, MA, USA), expression was detected using Alexa488- or Alexa594-conjugated secondary antibodies (Life Technologies).

Techniques: Cell Culture, Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Control